We will introduces the cyberinfrastructure, database and its applications in Qinghai Lake region. We have been building a cyberinfrastructure to support the protection and research in this area since 2006. We introduce the e-Science application practices supporting research on species identification, spatial distribution patterns analysis, and avian influenza virus cross-species infection by presenting their methods, implementation, and results. This is the first attempt in China to construct cyberinfrastructure to support protection and research on wild birds in plateau area. The e-Science application practices are also the first one applied in the national nature reserve in China.